Archives

  • 2019-10
  • 2019-11
  • 2020-03
  • 2020-07
  • 2020-08
  • br CONFLICTS OF INTEREST br The authors

    2020-08-18


    CONFLICTS OF INTEREST
    The authors have no conflicts of interest.
    ACKNOWLEDGMENTS
    We thank Sagetis Biotech for kindly providing oligopeptide-modified poly(b-amino ester)s (OM-pBAE) polymers. This work was devel-oped at the Centro Esther Koplowitz, Barcelona, Spain. M.R.-R. and G.R. are recipients of an FPI predoctoral contract (BES-2012-053726 and BES-2015-071612) from MINECO, Spain. This work was supported by grants to C.F. from the Spanish Ministry of Econ-omia y Competitividad (BIO2014-57716-C2-R and BIO2017-89754-C2-2R), with partial support from the Generalitat de Catalunya (SGR14/248, SGR17/861, and 2014/LLAVOR0061). CIBERER and CIBEREHD are an initiative of the ISCIII. The C.F. group was partially financed by the Instituto de Salud Carlos III (IIS10/00014) and co-financed by Fondo Europeo de Desarrollo Regional (FEDER); the C.F. group also acknowledges the support of COST Action BM1204 EUPancreas and the Spanish Adenovirus Network (AdenoNet, BIO2015-68990-REDT). We also acknowledge the sup-port of CERCA Programme/Generalitat de Catalunya. This work has been developed in the context of ADVANCE(CAT) with the sup-port of ACCIÓ (Catalonia Trade & Investment; Generalitat de Cata-lunya) and the European community under the Catalonian European Regional Development Fund operational program 2014–2020.
    REFERENCES
    4. Dorer, D.E., Holtrup, F., Fellenberg, K., Kaufmann, J.K., Engelhardt, S., Hoheisel, J.D., and Nettelbeck, D.M. (2011). Replication and virus-induced transcriptome of HAdV-5 in normal host Ursodeoxycholylglycine versus cancer cells–differences of relevance for adeno-viral oncolysis. PLoS One 6, e27934.
    Molecular Therapy
    7. van Beusechem, V.W., van den Doel, P.B., and Gerritsen, W.R. (2005). Conditionally replicative adenovirus expressing degradation-resistant p53 for enhanced oncolysis of human cancer cells overexpressing murine double minute 2. Mol. Cancer Ther. 4, 1013–1018.
    20. Ratovitski, E.A. (2014). Phospho-DNp63a/microRNA network modulates epigenetic regulatory enzymes in squamous cell carcinomas. Cell Cycle 13, 749–761.
    30. Subramanian, T., Zhao, L.J., and Chinnadurai, G. (2013). Interaction of CtBP with adenovirus E1A suppresses immortalization of primary epithelial cells and enhances virus replication during productive infection. Virology 443, 313–320.
    www.moleculartherapy.org
    48. Puig-Saus, C., Rojas, L.A., Laborda, E., Figueras, A., Alba, R., Fillat, C., and Alemany, R. (2014). iRGD tumor-penetrating peptide-modified oncolytic adenovirus shows enhanced tumor transduction, intratumoral dissemination and antitumor efficacy. Gene Ther. 21, 767–774. 
    51. Villanueva, E., Martí-Solano, M., and Fillat, C. (2016). Codon optimization of the adenoviral fiber negatively impacts structural protein expression and viral fitness. Sci. Rep. 6, 27546.
    Contents lists available at ScienceDirect
    Journal of Photochemistry & Photobiology, B: Biology
    journal homepage: www.elsevier.com/locate/jphotobiol
    Biosynthesis of selenium nanoparticles, characterization and X-ray induced T radiotherapy for the treatment of lung cancer with interstitial lung disease
    a Department of Oncology, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, PR China b Department of Clinical Laboratory, PLA 309 hospital, Beijing, PR China
    Keywords:
    Lung cancer
    SeNPs
    X-rays 
    Selinium nanoparticles (SeNPs) with minimal toxicity and efficient antioxidant properties were reported earlier for their anti-carcinogenic influence against various types of cancers, thus elevating its potential. In the present study, the anti-carcinogenic effect of selenium nanoparticles against lung cancer was studied. Selenium nano-particles were biosynthesized and were characterized using UV- Vis absorption spectroscopy. A decrease in the absorption intensity was recorded with the increase in time, which represented the protein consumption during the reduction of SeO32− to Se0. The calculated average crystalline size from XRD studies of the synthesized selenium nanoparticles was found to be 88.89 nm which was in accordance with the TEM analysis while the SAED pattern has disclosed hexagonal ring structure with diffraction ring pattern.MTT assay was performed to evaluate the radio-sensitizing effect of selenium nanoparticles under the X-ray influence against cancer as well as healthy cell lines. SeNPs showed potent cytotoxicity effect in cancer cells whereas it showed relatively less toxic effect in normal healthy cells. However, caspase-3 activity was even more elevated when subjected to X-ray exposure than in the absence. These findings apparently revealed the cytotoxic potential of SeNPs + X-ray combination in the Ursodeoxycholylglycine lung cancer cell lines.